
We have two types of real-time PCR kits: a closed kit using MiCo BioMed’s LabChip-type real-time PCR
and an open kit using universal tube-type ones
MiCo BioMed has been firmly standing at the forefront of the global
fight against COVID-19, providing comprehensive diagnostic solutions.
We have two types of real-time PCR kits: a closed kit using MiCo BioMed’s LabChip-type real-time PCR
and an open kit using universal tube-type ones
Veri-Q PREP M16 (MiCo BioMed)
Veri-Q PREP M16-16TU-CV19 kit (7A133, MiCo BioMed)
Veri-Q ManuPrep Viral DNA/RNA kit (7A231, MiCo BioMed)
Veri-Q PCR 316 (MiCo BioMed)
Veri-Q™ COVID-19 Multiplex Assay kit (nCoV-QM, MiCo BioMed)
QiaCube (Qiagen)
Veri-Q PREP M16 (MiCo BioMed)
QIAamp MinElute Virus Spin kit (57704, Qiagen)
Veri-Q PREP M16-16TU-CV19 kit (7A133, MiCo BioMed)
Veri-Q ManuPrep Viral DNA/RNA kit (7A231, MiCo BioMed)
Applied Biosystems™ 7500 (ABI 7500) Real-Time PCR System (Thermo Fisher)
CFX Connect Real-time PCR Detection System (Bio-Rad)
Veri-Q™ COVID-19 Multiplex Assay kit (nCoV-OM, MiCo BioMed)
Kits | Real-time PCR | ORF3a gene | N gene |
---|---|---|---|
nCoV-QM | Veri-Q PCR 316 | 8.9 copies/rxn | 9.0 copies/rxn |
nCoV-OM | CFX96 | 6.0 copies/rxn | 10.1 copies/rxn |
ABI 7500 | 21.6 copies/rxn | 21.9 copies/rxn |
Comparative Assay | Total | |||
---|---|---|---|---|
Positive | Negative | |||
nCoV-QM | Positive | 63 | 0 | 63 |
Potential positive | 1 | 0 | 1 | |
Negative | 1 | 93 | 94 | |
Total | 65 | 93 | 158 |
- Positive Percent Agreement (PPA):96.9 %[95% CI: 89.3, 99.6]
- Negative Percent Agreement (NPA):100 %[95% CI: 96.1, 100]
- Overall Percent Agreement (OPA):98.7 %[95% CI: 95.5, 99.8]
- Kappa: 0.97[95% CI: 0.94, 1]
Comparative Assay | Total | |||
---|---|---|---|---|
Positive | Negative | |||
nCoV-OM | Positive | 65 | 0 | 65 |
Negative | 0 | 93 | 93 | |
Total | 65 | 93 | 158 |
- Positive Percent Agreement (PPA):100 %[95% CI: 94.5,100]
- Negative Percent Agreement (NPA):100 %[95% CI: 96.1, 100]
- Overall Percent Agreement (OPA):100 %[95% CI: 97.7, 100]
- Kappa: 1[95% CI: 1, 1]
Specimen: nasopharyngeal or oropharyngeal swabs
Target genes: ORF3a & N genes
Kit unit: 100 tests per kit (95 X 40 X 55 mm / 70 g)
Running time for real-time PCR
- nCoV-QM: ~55 min using Veri-Q PCR 316 (MiCo BioMed)
- nCoV-OM: ~80 min using ABI (Thermo Fisher) or CFX96 (BioRad)